Quantitative PCR of Propionibacterium acnes DNA in samples aspirated from sebaceous follicles on the normal skin of subjects with or without acne

摘要: To elucidate whether people with hair follicles containing many Propionibacterium acnes cells are prone to acne, we developed a novel method count the number of P. in follicles. We sampled sebaceous material by aspiration at constant negative pressure from nose, forehead, and upper arm 86 patients acne vulgaris 209 control subjects healthy skin, including 84 age-matched patients. Genome-equivalents samples were estimated real-time quantitative PCR (TaqMan). Numbers genome-equivalents extremely low less than 10 years age generally higher greater ages, much variation same decade life. In men, median was highest controls aged 15-19 years; women, it peaked twice, again those 40 or older. counts on forehead nose 10-14 both sexes. The three sites similar 15 29 results suggest that not particularly except for younger teenagers. Our estimation can be used examine cutaneous microflora acnes.