Exploring diversity among Spanish strains of Erwinia amylovora and possible infection sources.

摘要: Aims:  We have examined the intraspecific diversity of a collection 63 Spanish strains Erwinia amylovora, isolated from 1995 to 2001, determine whether or not they could be grouped based on phenotypic genotypic criteria and investigate sources inoculum for fire blight dissemination in Spain. Methods Results:  Several biochemical molecular techniques, such as miniaturized API 20E, 50CH, ATB G-5 API-ZYM tests, BIOLOG metabolic fingerprinting, PCR ribotyping, pulsed-field gel electrophoresis (PFGE), minisatellite-primed (MSP-PCR), random amplified polymorphic DNA (RAPD) analyses AFLP were used. report first identification Spain PFGE pattern Pt1, already described other European countries, together with Pt3 Pt4 patterns. Moreover, PFGE, MSP-PCR, RAPD are, until now, only techniques that provided information about possible infection relationships between different foci Spain, being most discriminative. Conclusions:  These allowed grouping by their geographical origin. Significance Impact Study:  Our results support hypothesis some outbreaks been caused introduction infected plant material, countries.