GENOTYPING ERROR RATES ASSOCIATED WITH ALTERNATIVE SOURCES OF DNA FOR THE NORTH AMERICAN RIVER OTTER

摘要: The ability to accurately genotype individuals may depend upon interactions among numerous variables including the source of DNA, sample preservation method, reliability molecular marker being used, and species under study. use feces as an alternative DNA is becoming increasingly popular; however quality quantity often are compromised when using fecal material DNA. poor can introduce problems in downstream applications amplification failure genotyping errors. method used for collecting field known influence quality; however, decisions concerning storage mediums made independently information likelihood error. In this study we North American river otter (Lontra canadensis) scats anal jelly secretions sources microsatellite loci examine 5 methods on rates error species. occurrence 2 types errors (allelic dropout false alleles) was assessed relative both method. proportion also determined each locus investigate locus-specific rates. source, treatment, all influenced our obtain accurate genotypes, demonstrating need conducting preliminary studies before large- scale noninvasive genetic sampling efforts attempted. Baseline work must be performed prior collection any determine appropriate protocols minimization