Procedures to genotype problematic non-invasive otter ( Lutra lutra ) samples

摘要: Non-invasive genetics is a powerful tool in wildlife research and monitoring, especially when dealing with elusive rare species such as the Eurasian otter (Lutra lutra). Nevertheless, DNA obtained from scats anal secretions appears to be exposed very quick degradation processes, success rate amplification lower than other carnivores. We collected 191 samples April September 2011 along river Sangro basin (Italy) which was recently re-colonized by otter. Using two sets of microsatellite loci (six Lut seven OT loci), we investigated influence sample type age, collection time, storage temperature humidity on genotyping success. also tested efficacy different extraction kits buffer mediums. Finally, compared rate, allelic dropout false allele rates for each locus. mean 79.0 % 35.1 %. Fresh pure jellies yielded highest rate. Six should theoretically sufficient distinguish individual unrelated otters (PID = 0.001), while 13 were needed sibling (PIDsibs = 0.002) our population. identified 11 otters, molecular sexing ascertained presence five males, four females uncertain individuals. Generalized linear models highlighted significant both