Factors influencing genotyping success and genotyping error rate of Eurasian otter ( Lutra lutra ) faeces collected in temperate Central Europe

摘要: The use of non-invasively collected DNA source material for genetic and genomic applications is usually characterized by low target concentration quality, genotyping errors cost-intensive lab procedures. However, otters (Lutrinae) as elusive species conservation concern, non-invasive sampling has become an important tool to study their ecology demography. To increase cost-efficiency monitoring programmes promote the expansion approaches samples, we aimed refine sample collection preparation. Therefore, examined effects intrinsic characteristics (including diet), environmental conditions in field treatment molecular laboratory on success allelic dropout (ADO) rates using microsatellite markers 1970 fresh Eurasian otter (Lutra lutra) scats. Using samples only, probably eliminated one most impediments from faecal beforehand. But, observed higher lower ADO anal glad secretions containing high proportions mucus. Moist during may degradation PCR inhibition, leading decreased rates. was further affected type extraction kit. a proportion variance remaining unexplained our models implied that additional parameters were acting (amount inhibitors, non-uniform distribution intestinal cells, efficiency PCRs, specific microclimate at marking sites). We summarized influential factors maximizing quality scats give recommendations collection, storage based results current literature.