Severe Acute Respiratory Syndrome Coronavirus 2, COVID-19, and the Renin-Angiotensin System: Pressing Needs and Best Research Practices.

摘要: The coronavirus disease 2019 (COVID-19) pandemic is associated with significant morbidity and mortality throughout the world, predominantly due to lung cardiovascular injury. virus responsible for COVID-19-severe acute respiratory syndrome 2-gains entry into host cells via ACE2 (angiotensin-converting enzyme 2). a primary within key counter-regulatory pathway of renin-angiotensin system (RAS), which acts oppose actions Ang (angiotensin) II by generating Ang-(1-7) reduce inflammation fibrosis mitigate end organ damage. As COVID-19 spans multiple systems linked system, it imperative understand clearly how severe 2 may affect multifaceted RAS. In addition, recognition role RAS in has renewed interest its pathophysiology general. We provide researchers framework best practices basic clinical research interrogate using appropriate methodology, especially those who are relatively new field. This crucial, as there many limitations inherent investigating experimental models humans. discuss sound methodological approaches quantifying content activity (ACE, ACE2), peptides (Ang II, Ang-[1-7]), receptors (types 1 receptors, Mas receptor). Our goal ensure methodology investigations patients optimal rigor reproducibility interpretation results from these investigations.