An analysis of the phosphoproteome of immune cell lines exposed to the immunomodulatory mycotoxin deoxynivalenol.

摘要: Abstract The mycotoxin deoxynivalenol (DON) commonly contaminates cereal grains. It is ubiquitous in the Western European diet, although chronic, low-dose effects humans are not well described, but immunotoxicity has been reported. In this study, two-dimensional gel electrophoresis was used to identify phosphoproteomic changes human B (RPMI1788) and T (Jurkat E6.1) lymphocyte cell lines after exposure modest concentrations of DON (up 500 ng/mL) for 24 h. Proteins identified as having altered phosphorylation state post-treatment (C-1-tetrahydrofolate synthase, eukaryotic elongation factor 2, nucleoside diphosphate kinase A, heat shock cognate 71 kDa protein, translation initiation 3 subunit I growth receptor-bound protein 2) involved regulation metabolic pathways, biosynthesis signaling transduction. All exhibited a greater than 1.4-fold change, reproducible three separate experiments consisting 36 gels total. Flow cytometry validated observations 2 2. These findings provide further insights how low dose may affect immune function have potential mechanism-based phosphoprotein biomarkers exposure.