Heterozygous Cellular Glutathione Peroxidase Deficiency in the Mouse Abnormalities in Vascular and Cardiac Function and Structure

作者: Chee Chew Lim , John Jones , Pascal J. Goldschmidt-Clermont , Joseph Loscalzo , Marc A. Forgione

DOI: 10.1161/01.CIR.0000026820.87824.6A

关键词:

摘要: Background— Oxidant stress has been implicated in the pathogenesis of atherothrombosis and other vascular disorders accompanied by endothelial dysfunction. Glutathione peroxidases (GPx) play an important role cellular defense against oxidant utilizing glutathione (GSH) to reduce lipid hydroperoxides hydrogen peroxide their corresponding alcohols. Cellular GPx (GPx-1) is principal intracellular isoform GPx. We hypothesized that GPx-1 deficiency per se induces dysfunction structural abnormalities through increased stress. Methods Results— A murine model heterozygous (GPx+/−) was investigated examine this hypothesis. Mesenteric arterioles GPx-1+/− mice demonstrated vasoconstriction acetylcholine compared with vasodilation wild-type (maximal change vessel diameter, −13.0±2.8% versus 13.2±2.8%, P<0.0001). also noted increase plasma aortic levels isoprostane iPF2α-III, a marker ...

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