A pre-aspartate-specific protease from human leukocytes that cleaves pro-interleukin-1 beta
作者： R A Black , S R Kronheim , J E Merriam , C J March , T P Hopp
DOI: 10.1016/S0021-9258(18)83546-3
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摘要: Interleukin-1 beta is a 17.4-kilodalton hormone derived from 33-kilodalton inactive precursor produced by monocytes. We used the as substrate to detect proteolytic activities in peripheral blood mono-nuclear cell-conditioned medium that might be involved interleukin-1 processing. found conditioned medium, following passage through DEAE-Sephacel, generates biologically active fragment runs slightly higher than mature sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The responsible activity behaved single protein ion exchange chromatography. It was completely inhibited metal chelators and not inhibitors of serine, cysteine, or aspartate proteases, it dependent on both calcium (or magnesium) zinc. enzyme three substrate-based metalloprotease inhibitors, phosphoramidon, benzyloxycarbonyl-Gly-Leu-NH2, N-(2-carboxy-3-phenylpropionyl)-Leu. NH2-terminal sequence analysis showed cleavage occurred between histidine an residue, digestion synthetic peptides indicated protease specific for pre-aspartate cleavages.
sci-hub.st 参考文章(13)
C A Dinarello, P E Auron, G LoPreste, S F Mucci, H A Bernheim, J G Cannon, K J McAdam, S J Warner, L J Rosenwasser, A C Webb, Studies on the molecular nature of human interleukin 1. Journal of Immunology. ,vol. 138, pp. 1447- 1456 ,(1987)
R A Black, S R Kronheim, M Cantrell, M C Deeley, C J March, K S Prickett, J Wignall, P J Conlon, D Cosman, T P Hopp, Generation of biologically active interleukin-1 beta by proteolytic cleavage of the inactive precursor. Journal of Biological Chemistry. ,vol. 263, pp. 9437- 9442 ,(1988) , 10.1016/S0021-9258(19)76559-4
Charles A. Dinarello, Biology of interleukin 1. The FASEB Journal. ,vol. 2, pp. 108- 115 ,(1988) , 10.1096/FASEBJ.2.2.3277884
P. Matsudaira, Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. Journal of Biological Chemistry. ,vol. 262, pp. 10035- 10038 ,(1987) , 10.1016/S0021-9258(18)61070-1
D. Y. Mochizuki, J. R. Eisenman, P. J. Conlon, A. D. Larsen, R. J. Tushinski, Interleukin 1 regulates hematopoietic activity, a role previously ascribed to hemopoietin 1. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 84, pp. 5267- 5271 ,(1987) , 10.1073/PNAS.84.15.5267
Carl J. March, Bruce Mosley, Alf Larsen, Douglas Pat Cerretti, Gary Braedt, Virginia Price, Steven Gillis, Christopher S. Henney, Shirley R. Kronheim, Kenneth Grabstein, Paul J. Conlon, Thomas P. Hopp, David Cosman, Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs Nature. ,vol. 315, pp. 641- 647 ,(1985) , 10.1038/315641A0
P. E. Auron, A. C. Webb, L. J. Rosenwasser, S. F. Mucci, A. Rich, S. M. Wolff, C. A. Dinarello, Nucleotide sequence of human monocyte interleukin 1 precursor cDNA. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 81, pp. 7907- 7911 ,(1984) , 10.1073/PNAS.81.24.7907
S K Durum, J A Schmidt, J J Oppenheim, Interleukin 1: An Immunological Perspective Annual Review of Immunology. ,vol. 3, pp. 263- 287 ,(1985) , 10.1146/ANNUREV.IY.03.040185.001403
E K Bayne, E A Rupp, G Limjuco, J Chin, J A Schmidt, Immunocytochemical detection of interleukin 1 within stimulated human monocytes. Journal of Experimental Medicine. ,vol. 163, pp. 1267- 1280 ,(1986) , 10.1084/JEM.163.5.1267
Joost J. Oppenheim, Elizabeth J. Kovacs, Kouji Matsushima, Scott K. Durum, There is more than one interleukin 1. Immunology Today. ,vol. 7, pp. 45- 56 ,(1986) , 10.1016/0167-5699(86)90124-6