Development of microfluidic concentrator using ion concentration polarization mechanism to assist trapping magnetic nanoparticle-bound miRNA to detect with Raman tags.

摘要: MicroRNAs (miRNAs) are small noncoding single-stranded ribonucleic acid molecules. This type of endogenous oligonucleotide could be secreted into the circulation and exist stably. The detection specific miRNAs released by cancer cells potentially provides a noninvasive means to achieve early diagnosis prognosis cancers. However, typical concentration in blood is below ultratrace level. study uses simple thermoplastic microfluidic device based on an ion polarization mechanism perform enrichment cleanup Raman sensing beads determine miRNA quantitatively. One sample solution containing target molecules having been hybridized with two nucleotide probes, where one probe tag nanoaggregate embedded bead (NAEB) other magnetic nanoparticle (MNP), first filled device. When external field applied across cation exchange membrane stationed middle conduit device, MNP-miRNA-NAEB complexed particles enriched near edge cathode side. concentrated further trapped using magnet washing steps remove excess noncomplexed NAEBs. accomplished, remaining loaded capillary acquire signals from beads. Compared that conventional trapping time shortened nearly hour less than 10 min. Sample loss during becomes more controllable, resulting adequate standard curve linearity (R > 0.99) ranging 1 100 pM.