Preparation and characterization of green fluorescent nanodiamonds for biological applications

摘要: Abstract Green fluorescent nanodiamonds (denoted as gFNDs) have been produced and characterized for use cellular markers and/or labels. The gFND particles, containing a high concentration of N–V–N (or H3) centers, were prepared by radiation damage type Ia natural diamond nanocrystallites with either home-built 40-keV He + beam or research-grade 3-MeV H beam, followed thermal annealing at 800 °C. Prior to the irradiation treatment, infrared spectroscopy C–N stretches (at 1282 cm − 1 ) single crystals revealed nitrogen in range 900 ppm. Irradiation specimens led emergence broad absorption band ~ 470 nm, corresponding phonon sideband electronic transition H3 center. Measuring integrated coefficient zero-phonon line 503 nm) this center liquid temperature suggested density 1.7 × 10 18  centers/cm 3 10 ppm). A similarly defect centers was estimated treated under same experimental conditions. emitted green light ( λ em  = 530 nm) upon illumination blue laser ex  = 473 nm 488 nm). Applications novel nanomaterial demonstrated both confocal fluorescence microscopy flow cytometry 70-nm-sized particles uptaken live HeLa cells through endocytosis.