Effects of luteolin and quercetin, inhibitors of tyrosine kinase, on cell growth and metastasis-associated properties in A431 cells overexpressing epidermal growth factor receptor
作者： Y -T Huang , J -J Hwang , P -P Lee , F -C Ke , J -H Huang
关键词:
摘要: 1. Flavonoids display a wide range of pharmacological properties including anti-inflammatory. Anti-mutagenic, anti-carcinogenic and anti-cancer effects. Here, we evaluated the effects eight flavonoids on tumour cell proliferation, cellular protein phosphorylation, matrix metalloproteinase (MMPs) secretion. 2. Of examined, luteolin (Lu) quercetin (Qu) were two most potent agents, significantly inhibited A431 proliferation with IC50 values 19 21 micronM, respectively. 3. The epidermal growth factor (EGF) (10 nM) promoted cells (+25+/-4.6%) mediated receptor (EGFR) tyrosine kinase activity autophosphorylation EGFR by Lu Qu. At concentration 20 both Qu markedly decreased levels phosphorylation proteins, EGFR. 4. treated or exhibited protuberant cytoplasmic blebs progressive shrinkage morphology. also time-dependently induced appearance ladder pattern DNA fragmentation, this effect was abolished EGF treatment. 5. addition only marginally diminished inhibitory rate cells, treatment proteins greatly reduced indicating may act effectively to inhibit kinases, kinase. 6. increased metalloproteinase-2 (MMP-2) metalloproteinase-9 (MMP-9), while appeared suppress secretion these MMPs in cells. 7. Examination relationship between chemical structure reveal that double bond C2 C3 ring C OH groups C3' C4' B are critical for biological activities. 8. This study demonstrates Qu, stimulatory EGF, MMP be at least partly through supports idea have potential as anti-metastasis agents.
sci-hub.se 参考文章(62)
Thomas Krausz, Frances Balkwill, David Neal, Jonathan Waxman, Gordon Williams, David Thomas, Bernard Davies, Harpret Wasan, Andrew Hanby, Paul Abel, Levels of Matrix Metalloproteases in Bladder Cancer Correlate with Tumor Grade and Invasion Cancer Research. ,vol. 53, pp. 5365- 5369 ,(1993)
Joachim K�hnau, The flavonoids. A class of semi-essential food components: their role in human nutrition. World review of nutrition and dietetics. ,vol. 24, pp. 117- 191 ,(1976) , 10.1159/000399407
M S Hibbs, K A Hasty, J M Seyer, A H Kang, C L Mainardi, Biochemical and immunological characterization of the secreted forms of human neutrophil gelatinase. Journal of Biological Chemistry. ,vol. 260, pp. 2493- 2500 ,(1985) , 10.1016/S0021-9258(18)89580-1
Frances Balkwill, Kay Wilkinson, Rose Ann Smith, Nina-Beate Liabakk, Ian Talbot, Matrix Metalloprotease 2 (MMP-2) and Matrix Metalloprotease 9 (MMP-9) Type IV Collagenases in Colorectal Cancer Cancer Research. ,vol. 56, pp. 190- 196 ,(1996)
S. Cohen, H. Ushiro, C. Stoscheck, M. Chinkers, A native 170,000 epidermal growth factor receptor-kinase complex from shed plasma membrane vesicles. Journal of Biological Chemistry. ,vol. 257, pp. 1523- 1531 ,(1982) , 10.1016/S0021-9258(19)68224-4
Z Werb, J Gavrilovic, M J Banda, G S Herron, E J Clark, Secretion of metalloproteinases by stimulated capillary endothelial cells. II. Expression of collagenase and stromelysin activities is regulated by endogenous inhibitors. Journal of Biological Chemistry. ,vol. 261, pp. 2814- 2818 ,(1986) , 10.1016/S0021-9258(17)35860-X
T. Akiyama, J. Ishida, S. Nakagawa, H. Ogawara, S. Watanabe, N. Itoh, M. Shibuya, Y. Fukami, Genistein, a specific inhibitor of tyrosine-specific protein kinases. Journal of Biological Chemistry. ,vol. 262, pp. 5592- 5595 ,(1987) , 10.1016/S0021-9258(18)45614-1
Andreas Constantinou, Eliezer Huberman, Kaoru Kiguchi, Induction of differentiation and DNA strand breakage in human HL-60 and K-562 leukemia cells by genistein. Cancer Research. ,vol. 50, pp. 2618- 2624 ,(1990)
Hee-Jae Cha, Hiroshi Sato, Motoharu Seiki, Byung Chae Park, Soo-Kyung Bae, Kyu-Won Kim, Ho-Young Lee, Ok-Hee Lee, Anti-Invasive Activity of Ursolic Acid Correlates with the Reduced Expression of Matrix Metalloproteinase-9 (MMP-9) in HT1080 Human Fibrosarcoma Cells Cancer Research. ,vol. 56, pp. 2281- 2284 ,(1996)
Kristiina Wähälä, Lothar Schweigerer, Theodore Fotsis, Herman Adlercreutz, Michael S. Pepper, Roberto Montesano, Erkan Aktas, Sirpa Rasku, Stephen Breit, Flavonoids, dietary-derived inhibitors of cell proliferation and in vitro angiogenesis. Cancer Research. ,vol. 57, pp. 2916- 2921 ,(1997)