Akt Phosphorylates the Yes-Associated Protein, YAP, to Induce Interaction with 14-3-3 and Attenuation of p73-Mediated Apoptosis
作者： Subham Basu , Nicholas F Totty , Meredith S Irwin , Marius Sudol , Julian Downward
DOI: 10.1016/S1097-2765(02)00776-1
关键词:
摘要: We have used an affinity purification method to identify substrates of protein kinase B/Akt. One that associates with 14-3-3 in Akt-dependent manner is shown here be the Yes-associated (YAP), which phosphorylated by Akt at serine 127, leading binding 14-3-3. promotes YAP localization cytoplasm, resulting loss from nucleus where it functions as a coactivator transcription factors including p73. p73-mediated induction Bax expression following DNA damage requires function and attenuated phosphorylation YAP. overexpression increases, while depletion decreases, apoptosis damage, inhibitable manner. may thus suppress proapoptotic gene response cellular damage.
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