Involvement of Src kinase in T-type calcium channel-dependent neuronal differentiation of NG108-15 cells by hydrogen sulfide.
作者： Takeshi Tarui , Kazuki Fukami , Keita Nagasawa , Shigeru Yoshida , Fumiko Sekiguchi
DOI: 10.1111/J.1471-4159.2010.06774.X
关键词:
摘要: J. Neurochem. (2010) 114, 512–519. Abstract Hydrogen sulfide (H2S), a gasotransmitter, induces neuronal differentiation characterized by neuritogenesis and functional up-regulation of high voltage-activated Ca2+ channels, via activation T-type channels in NG108-15 cells. We thus analyzed signaling mechanisms for the H2S-evoked differentiation. NaHS, donor H2S, facilitated channel-dependent membrane currents, an effect blocked ascorbic acid that selectively inhibits Cav3.2 among three channel isoforms. applied once at concentration (13.5 mM) or repetitively relatively low (1.5 mM), as well ionomycin, ionophore, evoked neuritogenesis. The induced but not was abolished mibefradil, blocker. PP2, Src kinase inhibitor, completely suppressed caused NaHS while it only partially dibutyryl cAMP, inducer. actually phosphorylation Src, 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acetoxymethyl, intracellular chelator, mibefradil acid. currents cells treated with also inhibited PP2. Together, our data reveal participates NaHS/H2S
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