Archaeal and bacterial diversity in acidic to circumneutral hot springs in the Philippines.

摘要: The microbial diversity was investigated in sediments of six acidic to circumneutral hot springs (Temperature: 60-92 °C, pH 3.72-6.58) the Philippines using an integrated approach that included geochemistry and 16S rRNA gene pyrosequencing. Both bacterial archaeal abundances were lower high-temperature than moderate-temperature ones. Overall, community consisted sequence reads exhibited a high similarity (nucleotide identity > 92%) phyla Crenarchaeota, Euryarchaeota, unclassified Archaea. composed moderately related 90%) 17 phyla, with Aquificae Firmicutes being dominant. These phylogenetic groups correlated environmental conditions such as temperature, dissolved sulfate calcium concentrations spring water, sediment properties including total nitrogen, pyrite, elemental sulfur. Based on inference, sulfur metabolisms appear be key physiological functions these springs. Sulfobacillus (within phylum Firmicutes) along members within Sulfolobales abundant two (> 76 °C), they hypothesized play important role regulating cycling under conditions. results this study improve our understanding composition terrestrial their relationships geochemical