PfMyb1, a Plasmodium falciparum transcription factor, is required for intra-erythrocytic growth and controls key genes for cell cycle regulation.

摘要: During the complex life cycle of Plasmodium falciparum, divided between mosquito and human hosts, regulation morphologic changes implies a fine control transcriptional regulation. Transcriptional control, however, in particular its molecular actors, transcription factors regulatory motifs, are as yet poorly described Plasmodium. In order to decipher mechanisms implicated regulation, factor belonging tryptophan cluster family was studied. previous work, PfMyb1 protein, contained nuclear extracts, shown have DNA binding activity interact specifically with myb elements. We used long pfmyb1 double-stranded RNA (dsRNA) interfere cognate messenger expression. Parasite cultures treated dsRNA exhibited 40% growth inhibition when compared either untreated or unrelated dsRNA, parasite mortality occurred during trophozoite schizont transition. addition, transcript protein decreased by much 80% at time their maximum The global effect this partial loss investigated using thematic microarray encompassing genes involved signal transduction, cell SAM software enabled us identify several that were differentially expressed probably directly indirectly under PfMyb1. Using chromatin immuno-precipitation, we demonstrated binds, within nuclei, promoters therefore participates corresponding genes. This study provides first evidence network involving factor.