Incidence of Helicobacter pylori infection and their clarithromycin-resistant strains in otitis media with effusion regarding phenotypic and genotypic studies

摘要: Helicobacter pylori (H. pylori) are pathogenic bacteria that infect a half of the human population, colonize gastric mucosa and can be found in juice. Reflux juice has been suggested to associated with glue ear children. It tonsil adenoid tissues potential reservoirs H. infection. These observations raise question as whether infection might have role otitis media effusion (OME) The objectives this research were evaluate incidence possible pathogenesis OME children clarithromycin-resistant strains. Molecular assessment was done culture results vs. molecular study. A total 60 children, who prone ventilation tube insertion, adenoidectomy and/or tonsillectomy included control group consisted 40 underwent without history OME. Samples middle fluid mucosa, tissue, tonsillar tissue lavage cultured polymerase chain reaction (PCR) analysis then assembled by using QIAxcel System capillary electrophoresis for detection. There significant difference between cultures PCR (P < 0.05). Middle positive 40% patients 56.7% while 20% 26.7% results. Gastric 46.6% 63.3% patients. Adenoid 56.3% each, 70% 90%. presence lavage, significantly more frequent study compared group. minimum inhibitory concentration (MIC) values isolates ranged from 1.5 8 μg/ml. This showed around 50% revealed its sensitivity than techniques. clarithromycin resistance high among (39.6%).