Regulation of mating and filamentation genes by two distinct Ste12 complexes in Saccharomyces cerevisiae.

摘要: The Saccharomyces cerevisiae transcription factor Ste12 controls two distinct developmental programs of mating and filamentation. activity is regulated by Fus3 Kss1 mitogen-activated protein kinases through inhibitors, Dig1 Dig2. Mating genes are binding sites (pheromone response elements [PREs]), whereas filamentation supposedly the cooperative Tec1 on a PRE adjacent to Tec1-binding site (TCS), termed filamentous responsive element (FRE). However, most do not contain an FRE; instead, they all have TCS. By immunoprecipitation, we show that forms complexes, Ste12/Dig1/Dig2 Tec1/Ste12/Dig1, both in vivo vitro. complexes formed competitive Dig2 with Ste12, as can compete off from vitro vivo. In Tec1/Ste12/Dig1 complex, binds N terminus indirectly Ste12. has low basal activity, its transcriptional activation provided associated which under inhibition. Filamentation bound occupied mostly some Tec1/Ste12/Dig1. We suggest tethers TCS upstream defines subset Ste12-regulated genes.