Tryptophan degradation during heat treatments: Part 1—The degradation of free tryptophan

摘要: Abstract No tryptophan loss was observed when a 10 mM solution heated at 90 to 140°C for 1·5 24 h in the absence of oxygen. When thermal treatment carried out air or oxygen, degradation followed first order kinetics. The energy activation oxidative found approximate 80 000 J mole−1. rate increased with pH range 5–8. Up five products were detected by gel chromatography and be UV-absorbing but ninhydrin-negative. There good correlation between direct spectrofluorimetric HPLC-UV determinations tryptophan.