C-terminal heat shock protein 70 of Mycobacterium tuberculosis as a molecular adjuvant for DNA vaccination with the porcine circovirus type 2 ORF2 (capsid) gene in mice.
作者： Fang Fu , Huabin Tian , Xuesong Li , Yuekun Lang , Guangzhi Tong
DOI: 10.1016/J.TVJL.2012.06.005
关键词:
摘要: Mycobacterium tuberculosis heat shock protein 70 (HSP70) and the peptide binding C-terminal portion of HSP70 (amino acids 359-610; HSP70c) exert an adjuvant effect when used in vaccines. To enhance immunogenicity a DNA vaccine against porcine circovirus type 2 (PCV2), recombinant plasmids encoding PCV2 ORF2 (capsid) gene fused to full length hsp70 (pCA-TCH) or truncated hsp70c (pCA-TCHc) were constructed. Immunisation mice with pCA-TCHc induced higher serum immunoglobulin G antibody levels, stronger T helper 1 immune responses lower viral titres following challenge than immunisation pCA-TCH Cap only.
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