Transcriptional activation of the rat liver S14 gene during postnatal development.
作者： D B Jump , A Veit , V Santiago , G Lepar , L Herberholz
DOI: 10.1016/S0021-9258(18)68635-1
关键词:
摘要: The mRNA coding for the rat liver S14 protein (Mr 17,000, pI 4.9) shows profound increases during postnatal development. In an effort to define molecular basis rise in mRNAs14 we examined chromatin organization of gene, its DNA methylation state, hepatic expression mRNAs14, and vitro "run-on" activity prior after weaning at 21 days postpartum. animals less than or equal 15 age, gene is transcriptionally inactive, levels are 0.5% adult levels, within 11 kilobases 5' end similar that found tissues not expressing mRNAs14. From 18 22 postpartum, transcriptional greater 40-fold 100-fold approaching expression. Highly specific changes structure accompany activation. formation Hss-1 near cap site (-65 -265 base pairs) Hss-3 -3.3 upstream from suggests DNA-protein interaction these sites may function both tissue-specific developmental regulation studies suggest HhaI be a cue S14. However, maintenance hypermethylated HpaII throughout activation argues against role either These show principal mechanism accounting major development transcription stabilization RNA.
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