A liver-specific nuclear protein represses transcription of the S14 gene in vitro and in vivo.

摘要: P1 is a nuclear protein found exclusively in rat liver and binds to motif that spans nucleotides -310 -288 of the thyroid hormone responsive gene, S14. We expect play an important role regulating gene expression because binding for this factor contained within DNase I-hypersensitive site S14 chromatin. In report, we have attempted define function by correlating its DNA activity with levels mRNA-S14 response aging obesity. Results all studies revealed inverse relationships between mRNA-S14, thus suggesting may as repressor expression. Accordingly, tested hypothesis using cell-free transcription transient transfection assays measure reporter constructs without motif. presence motif, promoter was repressed negative effect on further enhanced hormone. These observations are consistent being transcription.