The Fate of Spermatogonial Stem Cells in the Cryptorchid Testes of RXFP2 Deficient Mice

摘要: The environmental niche of the spermatogonial stem cell pool is critical to ensure continued generation germ population. To study consequences an aberrant testicular environment in cryptorchidism we used a mouse model with deletion Rxfp2 gene resulting high intra-abdominal position. Mutant males were infertile gross morphology cryptorchid testis progressively deteriorating age. Few spermatogonia identifiable 12 month old testes. Gene expression analysis showed no difference between mutant and control testes at postnatal day 10. In three decrease (SSC) markers Id4, Nanos2, Ret was shown. direct counting ID4+ cells supported significant SSCs. contrast, Plzf, marker for undifferentiated differentiating not reduced, number PLZF+ higher testes, but equal six mutants. did show rate apoptosis testis. Sertoli FGF2 required SSC maintenance significantly reduced Based on these findings propose that deregulation somatic genes testis, directs SSCs towards differentiation pathway. This leads depletion increase cells, which too, eventually decreases exhaustion pool. Such dynamic suggests early correction retention