FRET-based aptamer probe for rapid angiogenin detection.
作者： Wei Li , Xiaohai Yang , Kemin Wang , Weihong Tan , Huimin Li
DOI: 10.1016/J.TALANTA.2007.12.012
关键词:
摘要: A sensitive method for rapid angiogenin (Ang) detection based on fluorescence resonance energy transfer (FRET) has been described. dual-labeled probe high affinity aptamer Ang was constructed. As donor and acceptor, 6-carboxyfluorescein (FAM) 6-carboxy-tetramethylrhodamine (TMR) were labeled at 5'- 3'-termini of the probe, respectively. The showed obvious changes due to specific binding between Ang. By monitoring intensity quantitative could be achieved. This assay is highly sensitive, with a limit 2.0 x 10(-10) mol L(-1) linear range 5.0 4.0 10(-8) in serum samples health lung cancer also detected.
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