Diagnosis of tuberculosis by DNA amplification in clinical practice evaluation

摘要: Various polymerase chain reaction (PCR) assays have been devised for the rapid identification of mycobacteria in clinical specimens. To assess value such routine laboratory work results obtained by PCR were compared with those standard microbiological methods 514 specimens collected investigation mycobacterial infection. Specimens tested presence Mycobacterium tuberculosis complex and atypical two assays, one based on amplification 65 kDa gene other IS6110 insertion sequence. For 489 samples that did not contain inhibitors findings correlated well bacteriological and/or data 476 (97.4%). 6 turned out to be false negatives, 3 positives 4 mis-identification strains. Pre-treatment guanidium thiocyanate reduced proportion false-negative contained inhibitors. This study confirms potential DNA early diagnosis infections.