Lactobacillus reuteri Maintains a Functional Mucosal Barrier during DSS Treatment Despite Mucus Layer Dysfunction

摘要: Treatment with the probiotic bacterium Lactobacillus reuteri has been shown to prevent dextran sodium sulfate (DSS)-induced colitis in rats. This is partly due reduced P-selectin-dependent leukocyte- and platelet-endothelial cell interactions, however, mechanism behind this protective effect still unknown. In present study a combination of culture dependent molecular based T-RFLP profiling was used investigate influence L. on colonic mucosal barrier DSS treated It first demonstrated that two mucus layers control animals had different bacterial community composition fewer bacteria resided firmly adherent layer. During induced colitis, number inner layer increased no longer differed. addition, induction dramatically altered microbial both loosely layers. Despite protecting against treatment did not improve integrity or distortion microbiota caused by DSS. However, decreased translocation from intestine mesenteric lymph nodes during treatment, which might be an important part mechanisms ameliorates colitis.