Mutagen testing using TRP+ reversion in Escherichia coli.
作者： M.H.L. Green , W.J. Muriel
DOI: 10.1016/0165-1161(76)90076-5
关键词:
摘要: Escherichia coli strain WP2 and its repair-deficient derivatives are suitable strains for mutagen screening. In these strains, agents which cause base substitution mutations can be shown to increase the frequency of Trp+ revertants. addition, causing many types DNA damage detected through increased killing repair deficient derivatives. Four ways performing tests described: (a) Spot in a small amount agent under test is placed directly on selective agar plate. revertants counted sensitivity determined from size zone inhibition cell growth. (b) Treat plate tests, where treated with subsequently plated determine survival or (c) A simplified fluctuation shows exceptional measuring mutation low levels mutagens. (d) Use liver microsomal fraction conjunction treat detect metabolically activated The merits defects systems discussed. Common pitfalls evaluating procedures avoiding them described.
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