ELISA solid phase: Partial denaturation of coating antibody yields a more efficient solid phase
作者： J.D. Conradie , M. Govender , L. Visser
DOI: 10.1016/0022-1759(83)90190-4
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摘要: Abstract We have examined conditions which will improve the solid phase in ELISAs for HBsAg and ferritin human serum. Sheep rabbit antibodies were used. It was found that pre-exposure of antibody to low pH, 3 M urea temperatures as high 82°C enhance ELISA colour these assays. Further experiments indicate perturb structure molecules results exposure new hydrophobic regions. is speculated more can bind regions on plastic surface normally not coated by non-perturbed molecules.
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