An improved preparation of antibody-coated polystyrene beads for sandwich enzyme immunoassay.
作者： Eiji Ishikawa , Yoshitaka Hamaguchi , Masayoshi Imagawa , Mitsuo Inada , Hiroo Imura
DOI: 10.1080/01971528008058479
关键词: Conformational change 、 Adsorption 、 Conjugate 、 Enzyme 、 Polystyrene 、 Antibody 、 Guinea pig 、 Immunoglobulin E 、 Chromatography 、 Chemistry
摘要: Abstract An improved preparation of antibody-coated polystyrene beads for sandwich enzyme immunoassay human thyroid-stimulating hormone (TSH) was described. Rabbit anti-TSH IgG purified by eluting at pH 2.5 from a TSH-Sepharose column, diluted 3 or 9 fold with normal rabbit and used coating physical adsorption. In inmunoassay TSH using (anti-TSH) Fab-β-D-galactosidase conjugate, β-D-galactosidase activities specifically bound to thus prepared in the presence 2.8–6.3 higher than those coated before purification. A similar effect observed when guinea pig anti-pork insulin IgG, (anti-human IgE) goat were treated 2.5. This improvement may be based on conformational change Fc molecule which caused treatment Other immunoassays such as fluoro-and radio-immunoassays also improve...
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