Kinetics of staphylococcal opsonization, attachment, ingestion and killing by human polymorphonuclear leukocytes: A quantitative assay using [3H] thymidine labeled bacteria
作者： Jan Verhoef , Phillip K. Peterson , Paul G. Quie
DOI: 10.1016/0022-1759(77)90141-7
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摘要: Abstract A method has been developed for studying quantitatively the separate processes of bacterial opsonization, phagocytosis, and killing by human polymorphonuclear leukocytes using [ 3 H]thymidine labeled Staphylococcus aureus . Phagocytosis is determined assaying leukocytes-associated radioactivity after differential centrifugation washing leukocytes. Opsonization studied incubating bacteria with an opsonic source varying durations then adding By treatment samples muralytic enzyme, lysostaphin, attachment ingestion phases phagocytosis can be separated. Sampling colony forming units disruption permits measurement killing. Using this method, differences in kinetics staphylococcal opsonization normal C2 deficient sera were defined, influences on delineated, different opsonins leukocyte populations determined.
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