Longitudinal study of circulating miR-122 in a rat model of non-alcoholic fatty liver disease

摘要: Abstract Background Circulating microRNAs (miRs) may be promising biomarkers for several diseases. We previously found that miR-122 can function as a biomarker non-alcoholic fatty liver disease (NAFLD). However, little is known regarding the time course of circulating levels during development NAFLD. Here, we examined using rat model Methods To clarify changes in serum NAFLD, experimental rats were fed high-fat diet (HFD) 2–10 weeks, while control received standard chow. Serum and tissue was collected from all animals at 2, 6, 10 weeks feeding. Clinical laboratory parameters (cholesterol, TG, AST, ALT, NEFA) determined by biochemistry analyzer. Hepatic lipid accumulation estimated Oil red O staining. then measured real-time polymerase chain reaction. Results Over feeding, body weight, total lipids, triacylglycerol increased HFD group compared to group. no significant alanine aminotransferase activity observed, suggesting NAFLD status mild. In contrast, observed drastic up-regulation levels. Our findings suggest level indeed useful assessing early might superior clinical markers traditionally used monitor hepatic disease.