Influence of irradiation on the osteoinductive potential of demineralized bone matrix

摘要: Samples of demineralized bone matrix (DBM) were exposed to graduated doses radiation (1–15 Megarad) (Mrad) utilizing a linear accelerator and then implanted into the thoracic region Long-Evans rats. Subcutaneous implantation DBM allogenic rats induces endochondral bone. In response implantation, cascade events ensues; mesenchymal cell proliferation on day 3 postimplantation, chondrogenesis 7, calcification cartilagenous chondrolysis 9, osteogenesis 11 resulting in formation an ossicle containing active hemopoietic tissue. Bone was assessed by measuring alkaline phosphatase activity, rate mineralization determined measuring45Ca incorporation mineral, and40Ca content measured extent mineralization; acid activity used as parameter for resorption. The dose (2.5 Mrad) currently banks sterilization tissue did not destroy induction properties DBM. Furthermore, 3–5 Mrad even enhanced induction, insofar it produced more at same interval time than obtained from unirradiated control samples. None these experiments abolished although induced irradiated with higher 5 delayed.