Rigid porous polyacrylamide-based monolithic columns containing butyl methacrylate as a separation medium for the rapid hydrophobic interaction chromatography of proteins.
作者： Shaofeng Xie , Frantisek Svec , Jean M.J Fréchet
DOI: 10.1016/S0021-9673(97)00254-9
关键词:
摘要: Abstract Macroporous poly(acrylamide-co-butyl methacrylate-co-N,N′-methylenebisacrylamide) monoliths containing up to 15% butyl methacrylate units have been prepared by direct polymerization within the confines of HPLC columns. The hydrodynamic and chromatographic properties these 50 mm×8 mm I.D. columns – such as back pressure at different flow-rates, effect percentage hydrophobic component in mixture, salt concentration on retention proteins, dynamic loading capacity, recovery were determined under conditions typical interaction chromatography. Using monolithic column, five proteins easily separated only 3 min.
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