Monolithic poly(glycidyl methacrylate-co-divinylbenzene) capillary columns functionalized to strong anion exchangers for nucleotide and oligonucleotide separation.
作者： Wolfgang Wieder , Clemens P. Bisjak , Christian W. Huck , Rania Bakry , Guenther K. Bonn
关键词: Glycidyl methacrylate 、 Theoretical plate 、 Copolymer 、 Van Deemter equation 、 Chemical engineering 、 Analytical chemistry 、 Chemistry 、 Monolith 、 Monolithic HPLC column 、 Divinylbenzene 、 Ion chromatography
摘要: In the present work, poly(glycidyl methacrylate-co-divinylbenzene) monoliths were synthesized and further derivatized to obtain strong anion exchange supports. Capillary (65 x 0.2 mm id) prepared in situ by copolymerization of glycidyl methacrylate divinylbenzene, employing 1-decanol tetrahydrofuran as porogens. The free epoxy groups a two step synthesis quaternary ammonium functionalities. On testing pressure stability monolith, highly linear dependence between flow rate drop was obtained, indicating high material even at rates. morphology copolymer investigated scanning electron microscopy. Mercury intrusion porosimetry showed narrow pore size distribution, having maximum 439 nm. recording van Deemter plot number theoretical plates per meter found be 59324. produced turned out suitable for separation nucleotides oligonucleotides.
sci-hub.se 参考文章(28)
D. Ishii, K. Asai, K. Hibi, T. Jonokuchi, M. Nagaya, A study of micro-high-performance liquid chromatography Journal of Chromatography A. ,vol. 144, pp. 157- 168 ,(1977) , 10.1016/S0021-9673(00)99351-8
Timothy A. Nieman, Douglas A. Skoog, F. James Holler, Principles of Instrumental Analysis ,(1971)
Hong Guan-Sajonz, Georges Guiochon, Evelyn Davis, Kim Gulakowski, David W Smith, Study of the physico-chemical properties of some packing materials. III. Pore size and surface area distribution Journal of Chromatography A. ,vol. 773, pp. 33- 51 ,(1997) , 10.1016/S0021-9673(97)00201-X
Shaofeng Xie, Frantisek Svec, Jean M.J Fréchet, Rigid porous polyacrylamide-based monolithic columns containing butyl methacrylate as a separation medium for the rapid hydrophobic interaction chromatography of proteins. Journal of Chromatography A. ,vol. 775, pp. 65- 72 ,(1997) , 10.1016/S0021-9673(97)00254-9
Shuhong Zhang, Jian Zhang, Csaba Horváth, Capillary electrochromatography of proteins with polymer-based strong-cation-exchanger microspheres Journal of Chromatography A. ,vol. 965, pp. 83- 92 ,(2002) , 10.1016/S0021-9673(01)01544-8
James L. Manganaro, Bruce S. Goldberg, Protein Purification with Novel Porous Sheets Containing Derivatized Cellulose Biotechnology Progress. ,vol. 9, pp. 285- 290 ,(1993) , 10.1021/BP00021A007
C. Viklund, F. Svec, J.M.J. Frechet, K. Irgum, Fast ion-exchange HPLC of proteins using porous poly(glycidyl methacrylate-co-ethylene dimethacrylate) monoliths grafted with poly(2-acrylamido-2-methyl-1-propanesulfonic acid) Biotechnology Progress. ,vol. 13, pp. 597- 600 ,(1997) , 10.1021/BP9700667
Michael Lämmerhofer, Frantisek Svec, Jean M.J. Fréchet, Wolfgang Lindner, Capillary electrochromatography in anion-exchange and normal-phase mode using monolithic stationary phases. Journal of Chromatography A. ,vol. 925, pp. 265- 277 ,(2001) , 10.1016/S0021-9673(01)01034-2
Krishna Kalghatgi, Csaba Horváth, Rapid peptide mapping by high-performance liquid chromatography. Journal of Chromatography A. ,vol. 443, pp. 343- 354 ,(1988) , 10.1016/S0021-9673(00)94806-4
David Sýkora, Frantisek Svec, Jean M.J Fréchet, Separation of oligonucleotides on novel monolithic columns with ion-exchange functional surfaces. Journal of Chromatography A. ,vol. 852, pp. 297- 304 ,(1999) , 10.1016/S0021-9673(99)00004-7