Capillary electrochromatography in anion-exchange and normal-phase mode using monolithic stationary phases.

摘要: Hydrophilic macroporous weak and strong anion-exchange stationary phases have been prepared in a monolithic format within untreated fused-silica capillaries by the simple thermally or UV-initiated polymerization of 2-dimethylaminoethyl methacrylate, 2-hydroxyethyl methacrylate ethylene dimethacrylate presence binary porogenic mixture dodecanol cyclohexanol. The tertiary amino functionalities were then alkylated situ to afford anion-exchangers. These new with optimized porous properties used for CEC separation various organic anions. Thus, 2-substituted propionic acid drugs (profens) was separated 13 min high column efficiencies up 231,000 plates/m achieved. substituted benzoic acids indicates that selectivity results primarily from interactions, while electrophoretic migration contributes only slightly. In addition, these hydrophilic anion-exchangers are also able separate weakly acidic, neutral basic compounds such as phenols, xanthines aromatic amines normal-phase electrochromatographic mode.