Neoglycoenzymes: a versatile tool for lectin detection in solid-phase assays and glycohistochemistry.

摘要: Carbodiimide-mediated coupling of p-aminophenyl glycosides to a naturally nonglycosylated enzyme yields neoglycoenzyme. This compound combines inherent enzymatic activity with synthetically conferred ligand properties lectins. Appropriate choice the allows custom-made synthesis reliably detect various types To exemplify practical applications this class compounds, glycosylated bacterial β-galactosidase has been employed quantitate plant lectins, immobilized on plastic surfaces as well nitrocellulose. Competitive inhibition by specific sugar ascertained dependence binding protein-carbohydrate interactions. In view lectins tools, sandwich lectin-binding assay for high mannose-type glycoprotein detection modified principally facilitate wide application other lectin-reactive chains introducing addition lectin determination in solid-phase assays, neoglycoenzymes allow one glycohistochemically localize endogenous tissue prints and sections minimum number steps. nonradioactive, rapid, sensitive, convenient concept, based conjugation an maintenance its receptor-binding activity, may find extended beyond lectinology receptor analysis.