作者: Paul Workman , Paul A Clarke , Sandrine Guillard , Florence I Raynaud
DOI: 10.1038/NBT0706-794
关键词:
摘要: technology to select for DARPins with novel binding specificities. However, like thioredoxin, cannot be efficiently exported the periplasm using signal sequences encoded in commonly available cloning vectors, such as PhoA sequence, a model sequence that has been extensively studied, and PelB found many pET vectors (