CREB3 regulatory factor -mTOR-autophagy regulates goat endometrial function during early pregnancy.

摘要: In domestic ruminants, a receptive endometrium is crucial for successful pregnancy. Although many essential molecular modulators and pathways have been identified during early pregnancy, the precise mechanisms regulating goat endometrial function remains largely unknown. Here, we describe novel regulator whereby hormones increased CREB3 regulatory factor (CREBRF) expression act as potential activator of autophagy in epithelial cells (EECs) via mTOR pathway. Our results showed that knockdown CREBRF shCREBRF hampered EECs proliferation by S-phase cell cycle arrest significantly inhibited function. We also reported CREBRF-mTOR-autophagy pathway plays vital role function, with blockade rapamycin demonstrating on prostaglandin (PGs) secretion attachment EECs. Moreover, chloroquine pretreatment proved above conclusion. Collectively, our findings provide new insight into indicate central PGs attachment.