Development of mismatch amplification mutation assays for the differentiation of MS1 vaccine strain from wild-type Mycoplasma synoviae and MS-H vaccine strains.

摘要: Mycoplasma synoviae is an economically significant pathogen in the poultry industry, inducing respiratory disease and infectious synovitis chickens turkeys, eggshell apex abnormality chickens. Eradication, medication vaccination are options for controlling M. infection. Currently there two commercial, live, attenuated vaccines available against synoviae: temperature sensitive MS-H vaccine strain NAD independent MS1 strain. Differentiation of strains from field isolates essential during eradication programs. The present study provides melt-curve agarose gel based mismatch amplification mutation assays (MAMA) to discriminate wild-type isolates. on A/C single nucleotide polymorphism at nt11 a HIT family protein coding gene. melt- agarose-MAMAs reliably distinguish genotype isolate 102 template number/DNA sample. No cross-reactions with other avian species were observed. can be performed directly clinical samples they run simultaneously previously described MAMAs designed discrimination developed applicable laboratories limited facilities promote rapid, simple cost effective differentiation