RNA interference in keratinocytes and an organotypic model of human epidermis.

摘要: Gene silencing approaches afford investigators the ability to gain important insight into normal functional requirements of specific epidermal proteins and promise yield a powerful therapeutic means dampen level that are mutated or frequently overexpressed in skin disease. The efficient tractable delivery siRNAs keratinocytes is seminal this process. Here, we describe techniques for transient long-term representative gene product, namely desmoglein 1, primary human maintained as submerged cultures three-dimensional organotypic raft cultures. As complement epidermal-specific targeting strategies mice, these technical permit relatively rapid loss-of-function studies purely without some potential influences present situ, such an immune system vasculature.