Diagnosis of cell death induced by methylglyoxal, a metabolite derived from glycolysis, in Saccharomyces cerevisiae.

摘要: Methylglyoxal (MG) is a ubiquitous metabolite derived from glycolysis; however, this aldehyde kills all types of cell. We analyzed the properties MG-induced cell death budding yeast Saccharomyces cerevisiae. The MCA1 gene encodes caspase homologue that involved in H2O2-induced apoptosis yeast, although disruption did not repress sensitivity to MG. In addition, intracellular oxidation level increase under conditions which MG Furthermore, genes encoding antioxidant enzymes affect susceptibility Here, we demonstrate cells killed by do exhibit characteristics TUNEL assay or an annexin V staining, but show those necrosis upon propidium iodide staining. at high concentrations provokes necrotic without generation reactive oxygen species S.