Expression of the Glyoxalase I Gene of Saccharomyces cerevisiae Is Regulated by High Osmolarity Glycerol Mitogen-activated Protein Kinase Pathway in Osmotic Stress Response

摘要: Methylglyoxal is a cytotoxic metabolite derived from dihydroxyacetone phosphate, an intermediate of glycolysis. Detoxification methylglyoxal performed by glyoxalase I. Expression the structural gene I (GLO1) Saccharomyces cerevisiae under several stress conditions was investigated using GLO1-lacZ fusion gene, and expression GLO1 found to be specifically induced osmotic stress. The Hog1p one mitogen-activated protein kinases (MAPKs) in S. cerevisiae, both Msn2p Msn4p are transcriptional regulators that thought control Hog1p-MAPK. completely repressed hog1Delta disruptant approximately 80 50% msn2Delta msn4Delta disruptants, respectively. A double mutant MSN2 MSN4 unable induce highly conditions. Glucose consumption increased 30% during adaptive period wild type strain. On contrary, it reduced 15% mutant. When yeast cell exposed conditions, glycerol synthesized as compatible solute. Glycerol glucose, rate-limiting enzyme biosynthesis glycerol-3-phosphate dehydrogenase (GPD1 product), which catalyzes reduction phosphate 3-phosphate. GPD1 also phosphate; therefore, induction scavenge methylglyoxal, production for adaptation