ALK-Rearranged Lung Cancer in Chinese: A Comprehensive Assessment of Clinicopathology, IHC, FISH and RT-PCR

摘要: Approximately 3–7% of non-small cell lung cancers harbor an anaplastic lymphoma kinase (ALK) gene fusion, constituting a new molecular subtype cancer that responds to crizotinib, ALK inhibitor. Although previous studies have evaluated ALK-rearranged cancers, the comprehensive analysis in Chinese has not well assessed. Herein, we identified 44 cases samples by fluorescent in-situ hybridization (FISH), immunohistochemistry (IHC), and reverse transcription polymerase chain reaction (RT-PCR) large number surgically resected cancers. All were adenocarcinomas, with 2 having additional focal squamous components. The goal was analyse clinicopathological features adenocarcinomas. Our data showed cribriform structure, prominent extracellular mucus any type mucous pattern may be either sensitive or specific predict rearrangement. We used FISH as standard detection method. compared rearrangement accuracy FISH, RT-PCR IHC. could define both fusion partner variant, but seemed unable detect all translocations involving gene. It is noteworthy IHC using D5F3 antibody (Cell Signaling Technology) higher sensitivity specificity than ALK1 (Dako). Therefore, conclude remains cost-effective efficient technique for diagnosing rearrangements can optimal screening clinical practice.