Multiplex Reverse Transcription-PCR Screening for EML4-ALK Fusion Transcripts

作者: Kengo Takeuchi , Young Lim Choi , Manabu Soda , Kentaro Inamura , Yuki Togashi

DOI: 10.1158/1078-0432.CCR-08-1018

关键词: ALK Gene RearrangementFluorescence in situ hybridizationMolecular biologyReverse transcription polymerase chain reactionExonLung cancerGene rearrangementBiologyMultiplexEML4/ALK Fusion Gene

摘要: … labeled green and red, respectively. … red, respectively. In this assay, the normal ALK locus would be expected to yield an overlapping signal, whereas a pair of separate green and red …

参考文章(22)
L L Lanier, A Miyajima, G P Nolan, A Shibuya, Y Morikawa, T Kitamura, J Phillips, S Kinoshita, M Onishi, D M Gorman, Applications of retrovirus-mediated expression cloning. Experimental Hematology. ,vol. 24, pp. 324- 329 ,(1996)
William D. Travis, Pathology and genetics of tumours of the lung , pleura, thymus and heart Published in <b>2004</b> in Lyon by IARC. ,(2004)
Karen Pulford, Laurence Lamant, Stephan W. Morris, Lisa H. Butler, Katrina M. Wood, David Stroud, Georges Delsol, David Y. Mason, Detection of Anaplastic Lymphoma Kinase (ALK) and Nucleolar Protein Nucleophosmin (NPM)-ALK Proteins in Normal and Neoplastic Cells With the Monoclonal Antibody ALK1 Blood. ,vol. 89, pp. 1394- 1404 ,(1997) , 10.1182/BLOOD.V89.4.1394
K. Pulford, S.W. Morris, F. Turturro, Anaplastic lymphoma kinase proteins in growth control and cancer. Journal of Cellular Physiology. ,vol. 199, pp. 330- 358 ,(2004) , 10.1002/JCP.10472
Justus Duyster, Ren-Yuan Bai, Stephan W Morris, Translocations involving anaplastic lymphoma kinase (ALK) Oncogene. ,vol. 20, pp. 5623- 5637 ,(2001) , 10.1038/SJ.ONC.1204594
Laurence Lamant, Randy D. Gascoyne, Marie Mich�le Duplantier, Florence Armstrong, Ashraf Raghab, Mukesh Chhanabhai, Evica Rajcan-Separovic, Janie Raghab, Georges Delsol, Estelle Espinos, Non-muscle myosin heavy chain (MYH9): a new partner fused to ALK in anaplastic large cell lymphoma. Genes, Chromosomes and Cancer. ,vol. 37, pp. 427- 432 ,(2003) , 10.1002/GCC.10232
A. V. Galkin, J. S. Melnick, S. Kim, T. L. Hood, N. Li, L. Li, G. Xia, R. Steensma, G. Chopiuk, J. Jiang, Y. Wan, P. Ding, Y. Liu, F. Sun, P. G. Schultz, N. S. Gray, M. Warmuth, Identification of NVP-TAE684, a potent, selective, and efficacious inhibitor of NPM-ALK Proceedings of the National Academy of Sciences of the United States of America. ,vol. 104, pp. 270- 275 ,(2007) , 10.1073/PNAS.0609412103
Scott A. Tomlins, Bharathi Laxman, Saravana M. Dhanasekaran, Beth E. Helgeson, Xuhong Cao, David S. Morris, Anjana Menon, Xiaojun Jing, Qi Cao, Bo Han, Jindan Yu, Lei Wang, James E. Montie, Mark A. Rubin, Kenneth J. Pienta, Diane Roulston, Rajal B. Shah, Sooryanarayana Varambally, Rohit Mehra, Arul M. Chinnaiyan, Distinct classes of chromosomal rearrangements create oncogenic ETS gene fusions in prostate cancer Nature. ,vol. 448, pp. 595- 599 ,(2007) , 10.1038/NATURE06024
Arianna Donella-Deana, Oriano Marin, Luca Cesaro, Rosalind H. Gunby, Anna Ferrarese, Addolorata M. L. Coluccia, Carmen J. Tartari, Luca Mologni, Leonardo Scapozza, Carlo Gambacorti-Passerini, Lorenzo A. Pinna, Unique substrate specificity of anaplastic lymphoma kinase (ALK): development of phosphoacceptor peptides for the assay of ALK activity. Biochemistry. ,vol. 44, pp. 8533- 8542 ,(2005) , 10.1021/BI0472954
Young Lim Choi, Kengo Takeuchi, Manabu Soda, Kentaro Inamura, Yuki Togashi, Satoko Hatano, Munehiro Enomoto, Toru Hamada, Hidenori Haruta, Hideki Watanabe, Kentaro Kurashina, Hisashi Hatanaka, Toshihide Ueno, Shuji Takada, Yoshihiro Yamashita, Yukihiko Sugiyama, Yuichi Ishikawa, Hiroyuki Mano, Identification of Novel Isoforms of the EML4-ALK Transforming Gene in Non–Small Cell Lung Cancer Cancer Research. ,vol. 68, pp. 4971- 4976 ,(2008) , 10.1158/0008-5472.CAN-07-6158