Immunohistochemistry as a screening tool for ALK rearrangement in NSCLC: evaluation of five different ALK antibody clones and ALK FISH.

摘要: Aims ALK FISH analysis is used as the reference standard to demonstrate ALK rearrangements, which qualify patients with pulmonary adenocarcinomas for therapy inhibitors. The aim of this study was find screening antibody clones best positive and negative percentage agreement FISH. Methods results Three hundred three were evaluated stained five (5A4; D5F3; ALK1; ALK01; SP8) standardized detection systems. D5F3 additionally assessed using OptiView enhanced amplification system. found 14 cases (4.6%) that harboured rearrangements. These at all intensities 5A4. To identify rearranged among cases, we subsequently analysed immunohistochemically FISH. Conclusions D5F3 exclusively strong intensity, without a single false-positive or false-negative case. number subsequent analyses required would have decreased from 303 (−95.4%), reducing significantly time, work costs any loss diagnostic quality accuracy.