Biomolecular identification of allergenic pollen: a new perspective for aerobiological monitoring?

摘要: Background Accurate and updated information on airborne pollen in specific areas can help allergic patients. Current monitoring systems are based a morphologic identification approach, time-consuming method that may represent limiting factor for sampling network enhancement. Objective To verify the feasibility of developing real-time polymerase chain reaction (PCR) an alternative to optical analysis, as rapid, accurate, automated tool detection quantification allergenic taxa. Methods The traditional cetyl trimethyl ammonium bromide-based was modified DNA isolation from pollen. Taxon-specific sequences were identified via bioinformatics or literature searches PCR amplified matching taxa; products, complementary degenerate TaqMan probes developed. accuracy quantitative assay tested 3 plant species. Results setup extraction protocol allowed us achieve good-quality DNA. nuclear gene fragments sequenced. Designed primer pairs selected taxa, mostly at required classification level. Pollen properly even when collected routine aerobiological tape. Preliminary assays grains successfully performed test species mixes. Conclusions approach revealed promising results quantification, analyzing Future perspectives could concern development multiplex simultaneous different taxa same tube application high-throughput molecular methods.