A mutant of Escherichia coli conditionally defective in the synthesis of holo-[acyl carrier protein].
作者： Mary L Polacco , John E Cronan Jr , None
DOI: 10.1016/S0021-9258(19)69270-7
关键词:
摘要: Abstract Supplementation of Escherichia coli pantothenate auxotrophs with varying concentrations results in a concomitant variation the intracellular level CoA but has no effect on holo-[acyl carrier protein] (holo-[ACP]) (Alberts, A., and Vagelos, P. R. (1966) J. Biol. Chem. 241, 5201-5204). The 4-phosphopantetheine moiety is transferred by enzyme holo-[ACP] synthase to apo-[acyl (apo-[ACP]) form holo-[ACP], active lipid synthesis. We mutagenized an E. K12 auxotroph selected for mutants unable grow low concentration (0.25 microM) that grew much higher (25 microM). One these strains was completely deficient synthase. Reversion recombinational genetic analyses indicated mutant growth phenotype due altered activity. changes levels engendered manipulation supplementation were consistent deficiency activity cultures supplemented pantothenate. site lesion (called acpS) localized map.
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