Establishing method of liver cancer chick chorioallantoic membrane M-CDX model

摘要: The invention relates to the field of life science, in particular an establishing method a liver cancer chick chorioallantoic membrane M-CDX model. comprises steps embryo eggs incubator for incubation 2-4 days, wherein temperature is 35 DEG C-40 C and humidity 60-80%, cutting top ends into 1cm *1cm window; doping (0.5-8) * 106 quantity cells extracellular matrixes prepare cell suspension, dropping suspension silicon rubber ring pre-placed on C, 50%-65% carrying out microscopic examination shooting. According invention, by taking as model, model transplantation objects constructed; carrier bearing layer with rich blood vessels; compared traditional advantaged short experiment period, low feeding cost convenient operate; required operation device devices are simple easy get, cost, common laboratories can be normally equipped devices; production processes operate without long-term experience accumulation.