In vitro assessment of environmental stress of persistent organic pollutants on the Indo-Pacific humpback dolphin

摘要: Persistent organic pollutants (POPs) are detected ubiquitously and linked to range of adverse health effects. The Indo-Pacific humpback dolphin inhabited the Pearl River Estuary (PRE), China, where high concentrations POPs have been reported. This study evaluated threats posed by in environment using an vitro system. We selected BNF(β-naphthoflavone) four (DDTs (dichlorodiphenyltrichloroethanes), CHLs(chlorides), HCHs(hexachlorocyclohexanes) HCB(hexachlorobenzene)) which had accumulated with treat cultured skin fibroblast cells (ScSF cells) dolphin, investigated expression patterns ecological stress biomarkers CYP1A1, AHR HSP70 cell line. results showed that CYP1A1 was up-regulated after being exposed different BNF, DDTs HCHs. CHLs, HCHs HCB promoted expression. increased BNF DDTs. Moreover, comet assay experiments revealed produced higher degree DNA damage ScSF than other POPs, implying PRE has threatened body, especially Our provided important information assess risk raised environmental vivo.