Understanding the impact of divalent cation substitution on hydroxyapatite: An in vitro multiparametric study on biocompatibility

摘要: Hydroxyapatite (HA), a stable and biocompatible material for bone tissue therapy, may present variable stoichiometry accept large number of cationic substitutions. Such substitutions modify the chemical activity HA surface, with possible impact on biocompatibility. In this work, we assessed effects calcium substitution diverse divalent cations (Pb2+, Sr2+, Co2+, Zn2+, Fe2+, Cu2+, or Mg2+) biological behavior HA. Physicochemical analyses revealed that apatite characteristics related to crystallinity dissolution/uptake rates are very sensitive nature substitution. Cytocompatibility was evaluated by mitochondrial activity, membrane integrity, cell density, proapoptotic potential, adhesion tests. With exception Zn-HA, all substituted HAs induced some level apoptosis. The highest apoptosis levels were observed Mg-HA Co-HA. Cu-HA only impair simultaneously density. relative densities after exposure modified while Co-HA significantly improved onto surface. These results show changes surface dissolution caused substitution, as well increase metal species released media, main responsible factors alterations © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 2011.